Anti-melanogenesis and anti-oxidant of Salix pseudo-lasiogyne water extract in α-MSH-induced B16F10 melanoma cells

Abstract

The inhibitory effects of Salix pseudo-lasiogyne water extract (SPWE) on melanogenesis were evaluated using intracellular tyrosinase activity and melanin content in B16F10 cells exposed to α-melanocyte-stimulating hormone (α-MSH). Meanwhile, the antioxidative capacity of SPWE was also investigated. SPWE suppressed intracellular tyrosinase activity and decreased the melanin content in B16F10 cells. The results showed that tyrosinase protein expression was suppressed by the SPWE-treated group. SPWE inhibited mRNA and protein expression of microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP)-1, and TRP-2. SPWE inhibited the intracellular reactive oxygen species level in B16F10 cells. SPWE not only demonstrated 1,1-diphenyl-2-picrylhydrazyl stable free radical (DPPH), and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-free radical scavenging activity but also possessed chelation capacity and ferric ions (Fe3+) reducing power. We concluded that anti-melanogenesis and antioxidant effect of SPWE may result from the down-regulation MITF and tyrosinase expression and the inhibition ROS generation. Overall, SPWE might be a potential candidate substance for whitening agent about pigmentation.