Cell culture media chronicle

Abstract

Cell culture media has been one of the key factors in cell culture technology since media supports cell survival and proliferation, as well as cellular functions. The quality and characteristics of a medium directly impact the research experimental results, the biopharmaceutical production rate, even the treatment outcomes of patients. Here we summarized the chronological development of balanced saline solution, natural media, synthetic media, classical media, serum free media, chemically define media, industrial media, protein free media, and animal component free media. This review has provided the information needed to facilitate investigators to decide an appropriate medium according to their aims.