Ti-plasmid vectors useful for functional analysis of rice genes

Abstract

We developed new binary vectors, pPZP2H-lac and pPZP2Ha3, for the transformation of rice plants. These binary vectors contain multiple cloning sites and the hygromycin B phosphotransferase gene as a plant - selectable marker. After Agrobacterium - mediated transformation, transgenic plants containing a T-DNA could be tightly selected by hygromycin B. The vectors are powerful tools for the functional analysis of genes.