Deletion of a rare fungal pks cgpks11 promotes chaetoglobosin a biosynthesis, yet defers the growth and development of chaetomium globosum

Abstract

We previously reported that chaetoglobosin A (ChA) exhibits a great potential in the bio-control of nematodes and pathogenic fungi. To improve the production of ChA, a CRISPR‐Cas9 system was created and applied for eliminating potential competitive polyketide products. One of the polyketide synthase encoding genes, Cgpks11, which is putatively involved in the biosynthesis of chaetoglocin A, was disrupted. Cgpks11 deletion led to the overexpression of the CgcheA gene cluster, which is responsible for ChA biosynthesis, and a 1.6‐fold increase of ChA. Transcription of pks‐1, a melanin PKS, was simultaneously upregulated. Conversely, the transcription of genes for chaetoglocin A biosynthesis, e.g., CHGG_10646 and CHGG_10649, were significantly downregu-lated. The deletion also led to growth retardation and seriously impaired ascospore development. This study found a novel regulatory means on the biosynthesis of ChA by CgPKS11. CgPKS11 af-fects chaetoglobosin A biosynthesis, growth, and development in Chaetomium globosum.