Bioassay-guided isolation of the major compound with antioxidant activity from the algerian medicinal plant bubonium graveolens

Abstract

Objective: The objective of this research was to isolate and identify the major compound from the antioxidant extract obtained from the leaves of Bubonium graveolens. Methods: A bioguided fractionation was done to isolate and identify the major compound responsible of the antioxidant activity. The chemical structure of the isolated natural compound was established by spectroscopic means including UV, Fourier transform infrared,1H NMR, and13C NMR. The purified compound was accurately tested for its antioxidant activity by the 2, 2-diphenyl-1-picrylhydrazyl assay at 517 nm. Results: The major compound named myricetin 3′-O-rhamnoside was isolated for the 1st time from the leaves of B. graveolens a medicinal specie of Algerian Sahara belonging to the Asteraceae family. The study showed that the myricetin 3ʹ-O-rhamnoside has an antioxidant potential 59% when compared with standard ascorbic acid with an IC50=0.916 mg/ml. Conclusion: In the present work, it was possible to isolate and identify for the 1st time the major compound from the antioxidant fraction of B. graveolens.