Improving salinity tolerance in Brassica (Brassica napus var. Bsa and Brassica campestris var. Toria) by exogenous application of proline and glycine betaine

Abstract

The pot culture experiment was conducted to determine the influence of proline and glycine betaine on Brassica under saline conditions. Different salinity levels (0, 65,130 mM) were created according to the saturation percentage of the soil. Proline (0, 4, 8 mM) and glycine betaine (0, 5, 10 mM) were exogenously applied to find out their effects on growth and physiological changes produced in Brassica under salinity stress. Salinity stress reduced the growth of the plants and induced the physiological and biochemical changes. Different growth parameters of plants such as plant height, shoot, root fresh and dry weight was decreased with the increase of salinity stress. Salinity has also reduced the chlorophyll content, protein content and nitrate reductase activity of the Brassica. But the application of proline and glycine betaine was more effective to reduce the effect of salinity. Collected data from the present experiment indicated that adverse effects of salinity were counteracted by proline and glycine betaine. Overall, it was observed that exogenous application of both proline and glycine betaine has reduced the effect of salinity.