Antioxidant and anti-inflammatory effects of seed ethanol extracts of Rubus coreanus miquel

Abstract

This study investigated the antioxidant and anti-inflammatory effects of seed ethanol extracts from Rubus coreanus Miquel (SERC). To investigate the antioxidant activity, total polyphenol and flavonoid content, ABTS and DPPH radical scavenging activity, and reducing power were measured. The total polyphenol and flavonoid contents in seed ethanol extracts of R. coreanus Miq. were 4.09 μg gallic acid equivalents (GAE)/mg and 16.25 μg quercetin equivalents (QE)/mg, respectively. DPPH and ABTS radical scavenging activity showed concentration-dependent scavenging activity, and the RC50 values of SERC were 26.68 μg/mL and 39.30 μg/mL, respectively. Moreover, the ferric reducing antioxidant power (FRAP) assay was performed to assess the reducing power, and SERC showed 0.61 ± 0.01 mM FeSO4 E/mg. To measure the anti-inflammatory effect, the cytotoxicity and nitric oxide (NO) production inhibitory efficacy in RAW 264.7 cells was confirmed. SERC showed a NO production inhibitory effect at 500 μg/mL without cytotoxicity. As a result of verifying the antioxidant and anti-inflammatory activity using SERC, its potential as an antioxidant and anti-inflammatory material was confirmed.