Microsatellite Marker LEI0258 Variability in Six Indonesian Local Chicken Populations

Abstract

The microsatellite LEI0258 is a genetic marker for chicken MHC haplotypes and can be used to study the influence of population genetics on immune responses. In this study, we investigated the variability of the LEI0258 locus in 173 chickens from six Indonesian local chicken populations (Black Kedu Pelung, Sentul, Merawang, Nunukan, and Gaga). The LEI0258 locus was found to be polymorphic in overall populations with a total of 25 alleles. The observed and expected heterozygosity values ranged from 0.565 (Gaga) to 0.967 (Pelung) with an overall mean of 0.855 and from 0.715 (Gaga) to 0.879 (Merawang) with an overall mean 0.829, respectively. The decreasing heterozygosity indicated that the population's genetic diversity had decreased. The average PIC values ranged from 0.687 (Gaga) to 0.870 (Merawang), overall studied chicken was 0.811. All the chicken populations were in Hardy Weinberg equilibrium, except for the Gaga chicken.