Smith-degradative studies on the polysaccharide portion of A-band lipopolysaccharide from a mutant (AK1401) of Pseudomonas aeruginosa strain PAO1

Abstract

Hepta-O-acetyl-O-α-D-Rhap-(1 → 3)-O-α-D-Rhap-(1 → 2)-glycerol (5) was the major component derived by way of Smith degradation of A-band polysaccharide (a D-rhamnan isolated from a mutant, AK1401, of Pseudomonas aeruginosa strain PAO1). The structure has been verified by an unambiguous synthesis of 5. Based on mass spectrometric evidence, hepta-O-acetyl-O-Ribp-(1 → 3)-O-α-D-Rhap-(1 → 2)-glycerol is considered to be one of the minor components. The Smith degradation of A-band polysaccharide and the synthesis of 5 are reported.