Effect of α,α-dialkyl amino acids on the protease resistance of peptides

Abstract

A tryptic [EC 3.4.21.4] digestion assay of 2-aminoisobutyric acid (Aib)-containing peptides was carried out to investigate the effect of α,α-dialkyl amino acid residues on the protease resistance. The introduction of Aib residues to the P1′ positions exhibited a 19-fold higher protease resistance than the peptide with Aib residues introduced to the P2 position or the non-Aib peptide. The peptide having Aib residues introduced to the P1′ and P2 positions resulted in complete resistance.