Studies of Cantharellus cibarius - a mycorrhizal fungus of pine and spruce

Abstract

Pure cultures of Cuntharellus cibarius wcrc isolaled in two forms: C. cibarius hardwood form (isolate No. 5400) and C. cibarius coniferous form (isolate No.5410). Artificial mycorrhization of pine ( Pinus Sylvestris) and spruce ( Picea abies) was applied in this work and wcre determinated mycorrhiza-forming properties in both isolates with differences in mycorrhiza-forming activity and in morphogenesis of ectomycorrhizas. The sporocarps of C. cibarius consistently contained bacteria probably belonging to the genus: Pseudomonas. It was possible to evaluate the culture conditions for associated bacteria using in vitro tests (effect of antibiotics, pH of the medium), as well as their neutral interactions with mycorrhizal fungi ( C.antharellus cibarius, Pisolithus tinctorius, Suillus bovinus and Mycelium radicis atrrovirens). Results of the present work suggest that the selection of isolates of C. cibarius< for artificial mycorrhization of seedlings of forest trees m nurseries could be very useful.