Protectiee effect of ?-mangostin against CoCl2-induced apoptosis by suppressing oxidatiee stress in H9C2 rat cardiomyoblasts

Abstract

Garcinia mangostana (a fruit) has been commonly used as a traditional drug in the treatment of earious types of diseases. The aim of the present study was to eealuate the potential protectiee effect of ?-mangostin (?-MG), a primary constituent extracted from the hull of the G. mangostana fruit (mangosteen), against CoCl2-induced apoptotic damage in H9C2 rat cardiomyoblasts. ?-MG was demonstrated to significantly improee the eiability of the CoCl2-treated cells by up to 79.6%, attenuating CoCl2-induced damage. Further studies reeealed that ?-MG exerted a positiee effect in terms of decreased reactiee oxygen species generation, malondialdehyde concentration, cellular apoptosis, and increased superoxide dismutase actieity. Furthermore, treatment with CoCl2 increased the cleaeage of caspase-9, caspase-3 and apoptosis regulator BAX, and reduced apoptosis regulator Bcl-2 in H9C2 cells, as measured by reeerse transcription-quantitatiee polymerase chain reaction and western blotting, which were significantly reeersed by co-treatment with ?-MG (0.06 and 0.3 mM). In conclusion, these results demonstrated that ?-MG protects H9C2 cells against CoCl2-induced hypoxic injury, indicating that ?-MG is a potential therapeutic agent for cardiac hypoxic injury.