T-type calcium current contributes to escape automaticity and governs the occurrence of lethal arrhythmias after atrioventricular block in mice

Abstract

Background-When complete atrioventricular block (AVB) occurs, infranodal escape rhythms are essential to prevent bradycardic death. The role of T-type Ca2+ channels in pacemaking outside the sinus node is unknown. We investigated the role of T-type Ca2+ channels in escape rhythms and bradycardia-related ventricular tachyarrhythmias after AVB in mice. Methods and Results-Adult male mice lacking the main T-type Ca2+ channel subunit Cav3.1 (Cav3.1-/-) and wild-type (WT) controls implanted with ECG telemetry devices underwent radiofrequency atrioventricular node ablation to produce AVB. Before ablation, Cav3.1-/- mice showed sinus bradycardia (mean±SEM; RR intervals, 148±3 versus 128±2 ms WT; P<0.001). Immediately after AVB, Cav3.1-/- mice had slower escape rhythms (RR intervals, 650±75 versus 402±26 ms in WT; P<0.01) but a preserved heart-rate response to isoproterenol. Over the next 24 hours, mortality was markedly greater in Cav3.1-/- mice (19/31; 61%) versus WT (8/26; 31%; P<0.05), and Torsades de Pointes occurred more frequently (73% Cav3.1-/- versus 35% WT; P<0.05). Escape rhythms improved in both groups during the next 4 weeks but remained significantly slower in Cav3.1 -/-. At 4 weeks after AVB, ventricular tachycardia was more frequent in Cav3.1-/- than in WT mice (746±116 versus 214±78 episodes/24 hours; P<0.01). Ventricular function remodeling was similar in Cav3.1-/- and WT, except for smaller post-AVB fractional-shortening increase in Cav3.1-/-. Expression changes were seen post-AVB for a variety of genes; these tended to be greater in Cav3.1-/- mice, and overexpression of fetal and profibrotic genes occurred only in Cav3.1 -/-. Conclusions-This study suggests that T-type Ca2+ channels play an important role in infranodal escape automaticity. Loss of T-type Ca2+ channels worsens bradycardia-related mortality, increases bradycardia-associated adverse remodeling, and enhances the risk of malignant ventricular tachyarrhythmias complicating AVB. © 2013 American Heart Association, Inc.