Differential responses of arbuscular mycorrhizal fungi to nitrogen addition in a near pristine Tibetan alpine meadow

Abstract

Elucidating the responses of soil microbial abundance and community composition to nitrogen (N) addition is important for predicting ecosystem function under increased atmospheric N deposition. We examined the arbuscular mycorrhizal (AM) fungal community under three N forms (NH4+-N, NO3--N, and NH4NO3-N) and two N rates (1.5 and 7.5 g N m-2 year-1) in an alpine meadow of the Qinghai-Tibetan Plateau. AM fungal extraradical hyphal density was significantly decreased by NH4+-N in May, but was not affected by N form nor N rate in August. N rate, but not N form, significantly affected AM fungal spore density; high N rate decreased spore density. No direct N addition effect was observed on AM fungal community; however, soil available phosphorus, pH, and NO3--N were considered as important factors that influenced AM fungal community composition. Structural equation model results showed that N rate, not N form, strongly affected soil characteristics, which directly influenced community compositions of plants and AM fungi, as well as spore density. Therefore, AM fungal community was influenced by N addition, primarily because of altered soil characteristics, and partially by a modified plant community, but not or just slightly by direct N addition effects in this alpine meadow ecosystem. Arbuscular mycorrhizal fungal community was more influenced by altered soil characteristics than by direct nitrogen addition in an alpine meadow.