An Embryonated Egg Transmission Model for Epizootic Hemorrhagic Disease Virus

Abstract

Epizootic hemorrhagic disease virus (EHDV) is a vector-borne orbivirus of ruminants; in North America there are three serotypes (EHDV-1, -2, and -6) and these primarily affect white-tailed deer (Odocoilus virginianus). EHDV is vectored by biting midges, Culicoides spp. Embryonated chicken eggs (ECE) have recently been used as an experimental host to investigate the vector competence of Australian Culicoides spp. for bluetongue serotype virus 1 and 23. In this study, we evaluated the use of the ECE model to determine its applicability for evaluating vector competence related to transmission of North American EHDV serotypes. We demonstrated that all three North American EHDV serotypes were able to replicate in ECEs and be transmitted from infected ECEs to Culicoides sonorensis Wirth & Jones. In addition, we were able to complete the transmission cycle from infected C. sonorensis to uninfected ECEs for EHDV-1 and -2.