Molecular cloning and analysis of cDNA sequences derived from polyA+ RNA from barley endosperm: Identification of B hordein related clones

Abstract

A collection of over 130 cDNA clones has been constructed in the bacterial plasmids pPH207 and pBR322 using as template the poly A+ RNA from membrane-bound polysomes of barley endosperm (cv. Sundance). Fifty four B hordein cDNA clones have been identified by cross-hybridization analysis and in vitro translation of plasmid-selected mRNAs. Hybridization of TT of the B hordein cDNA clones to Northern blots of sizefractionated RNA indicated that the B hordein mRNA is ca. 1300 nucleotides long. One cDNA clone, pHvE-cl6, has been partially sequenced and shown by comparison with C-terminal and other peptide sequences to be related to Bl hordein polypeptides. The results obtained from the analysis of the B hordein cDNA clones support the idea that the Hor 2 locus, which specifies the B hordeins, is complex and codes for a family of related mRNA species. © 1981 IRL Press Limited.