Isolation and identification of some uncommon bacterial species isolated from different clinical sample

Abstract

There are many opportunistic bacterial species that are uncommon and infrequently exist in clinical specimen, most of them are difficult to routine identification, even some of them are poorly documented in clinical specimen. also had no less role in the coordinates of the disease than common bacterial species. Six hundred and fifty samples were collected from patients attending to some hospitals in Sulaimanya City and Kalar General Hospital during the period from October 2015 to November 2016. Samples were firstly cultured on different media in order to isolate and identify bacterial isolates according to cultural characteristics, morphological features and biochemical reactions in addition to Vitek 2 system for identifying uncommon and infrequent isolates. The identification and susceptibility test were performed in Kalar General Hospital. Isolated 286(44%) bacterial strains from different clinical samples, 125 of them were identified by Vitek 2 automated system, while 23(8%) of isolates were considered as uncommon bacterial species. The antimicrobial susceptibility of uncommon isolates, showed significant variation against twenty four antibiotics. Four isolates; Acinatobacter baumanii, Acinatobacter calcoaceticus, Enterobacter ludwigii and Gemella sanguinis were resistant to all antibiotics. Whereas Aerococcus urinae, (Citrobacter freundii and Alloiococcus otitis), (Morganella morganii, Sphingomonas paucimobilis, Pantoea agglomerans, Streptococcus sobrinus and Kocuria rosea n.2), (Ochrobactrum anthropi n.1 and Kocura kristinae), (Ochrobactrum anthropi n.2 and Kocuria rosea n.1), (Pseudomonas stutzeri, Pseudomonas fluorescens and Citrobacter koseri), (Aeromonas salmonicida and Micrococcus luteus n.1) and Micrococcus luteus (n.2) resistant to the antibiotics as these percentages 95.8%, 91.6%, 87.5%, 83.3%, 79.1%, 70.8%, 66.6% and 62.5%, respectively.