In vitro evaluation of antioxidant activity of D-Limonene

Abstract

Objective: Reactive oxygen species (ROS) and free radicals are involved in pathogenesis of cancer. D-Limonene, a monoterpene present in citrus fruit has been reported for its potential anticancer activities. The aim of present study was to evaluate the in vitro antioxidant effect of D-Limonene. Material and methods: Test solutions with different concentration of D-Limonene and Trolox (standard) were prepared. Six different In vitro antioxidant assays such as DPPH, ABTS, FRAP, iron chelating, hydroxyl radical scavenging and superoxide radical scavenging assay were performed for evaluation of antioxidant activity of D-Limonene. Results: D-Limonene has shown appreciable antioxidant activity in comparison with Trolox. The IC of D-Limonene in DPPH ( 384.73 µM), ABTS ( 603.23 µM,), 50 FRAP ( -589.85 µM), iron chelating (-18475.5 µM), hydroxyl radical scavenging (442.75 µM) and superoxide radical scavenging assay (692.89 µM) was comparable with Trolox the IC value of Trolox DPPH ( 153.30 µM), ABTS 50 ( 203.37 µM,), FRAP (-171.73 µM), iron chelating (225.96 µM), hydroxyl radical scavenging (146.37 µM) and superoxide radical scavenging assay ( 105.25 µM) respectively. Conclusion: In vitro antioxidant assays has shown concentration dependent reduction in free radical formation by D-Limonene in comparison with Trolox in all assays except iron chelating assay which suggests its promising role for cancer treatment.