Applying weight gain in Pomacea lineata (SPIX 1824) (Mollusca: Prosobranchia) as a measure of herbicide toxicity.

Abstract

Pomacea lineata, an extremely ubiquitous snail and pest to rice farmers throughout Asia, holds promise as a valuable resource for monitoring water quality in northeast Brazil. In this paper, we present data demonstrating the rate of weight gain in P. lineata neonates as a consistent measure of the stress imposed by sublethal concentrations of the herbicides Paraquat and Round-up. Our secondary agenda is to demonstrate the feasibility of incorporating bioassay into the standard municipal and state procedure of monitoring water quality. Growth data to assess chronic toxicity were generated in experiments of four and four, eight, twelve and sixteen days for Paraquat and Round-up, respectively. We estimated a 96 h no observed effect concentration (NOEC) and lowest observed effect concentration (LOEC) for Paraquat of 0.12 and 0.25 mg/L. The 96 h Round-up data yielded NOEC and LOEC values, respectively, of 0.25 and 0.5 mg/L. All concentrations of Round-up tested for the 192 h exposure yielded significantly lower growth than the control. Consequently, no NOEC could be derived. The LOEC was < 0.12 mg/L. Furthermore, there was no mortality during the test. At the lowest concentrations of Paraquat tested (0.005 mg/L) there was a significant increase in growth compared with the controls, suggesting a hormetic effect.