Phytoconstituents Screening, TLC, And GC-MS Analysis Of Barleria Cristata Linn. Leaves Methanolic Extract

Abstract

Barleria cristata Linn is an ornamental plant that grows on the sides of roads in India, China, and other countries. This plant belongs to the family Acanthaceae. In India, it is used as herbal medicine and is called Kala Bansa. It has an ancient legacy of application in folk medicine, particularly for the treatment of tuberculosis and snake bites. The purpose of the present study was a preliminary phytochemical analysis, Thin-Layer Chromatography (TLC) analysis and Gas chromatography-mass spectroscopy (GCMS) analysis of the methanol extract of Barleria cristata Linn leaves. The preliminary phytochemical screening finding revealed compounds like Alkaloids, Glycosides, Flavonoids, Carbohydrates, Proteins, and amino acids, and Reducing sugars. Thin-layer chromatography (TLC) of the methanolic extract was executed for 5 essential phytoconstituents Alkaloids, Flavonoids, Saponins, Glycosides, and Triterpenoids.  The leaf extract of this plant was analyzed using GCMS. This report revealed the 15 compounds. The bioactive chemicals were found by comparing the retention time and peak area with the literature. Barleria cristata possess various medicinal properties, so more research must be done on its traditional claims.