Real-time PCR in Clinical Diagnostic Settings

Abstract

Real-time PCR is a commonly used technique for the detection of pathogens both in research setting, as well as in diagnostic settings. The goal of this editorial is to introduce or familiarize the reader with the advantages of using the available methodologies in a targeted manner. Real-time PCR combines PCR chemistry with either a fluorescent probe or DNA detection dyes such as syber green, thus allowing visualization of product in "real-time". The available real-time PCR methodologies provide good sensitivity and specificity. The ease and speed of the design and setup process of qPCR makes it an attractive assay to use. PCR amplification is performed in a closed setting (one tube vs two tube) thus reducing the chances of contamination.