Effect of extraction method on the chemical profiles and bioactivities of soybean hull polysaccharides

Abstract

The yields, properties, and bioactivities of polysaccharides extracted by three methods from soybean hulls (SSCPs) were evaluated: hot water extraction (H-SSCP), microwave-assisted ammonium oxalate extraction (A-SSCP), and microwave-assisted sodium citrate extraction (S-SSCP). A-SSCP gave the highest yield of polysaccharides (9.3 ± 0.5%) although all three products had similar physicochemical characteristics and FT-IR spectra. A-SSCP and S-SSCP produced polysaccharides with lower molecular weight distributions and higher total reducing power and scavenging ability for ABTS+• and DPPH• free radicals. Furthermore, the effect of SSCPs on carbon tetrachloride (CCl4)-induced liver injury was investigated in the mice. When compared with H-SSCP and S-SSCP, A-SSCP significantly decreased the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), and reactive oxygen (ROS) to normal (p