Notonesomycin A is a 32-membered bioactive glycosylated macrolactone known to be produced by Streptomyces aminophilus subsp. notonesogenes 647-AV1 and S. aminophilus DSM 40186. In a high throughput antifungal screening campaign, we identified an alternative notonesomycin A producing strain, Streptomyces sp. A793, and its biosynthetic gene cluster. From this strain, we further characterized a new more potent antifungal non-sulfated analogue, named notonesomycin B. Through CRISPR-Cas9 engineering of the biosynthetic gene cluster, we were able to increase the production yield of notonesomycin B by up to 18-fold as well as generate a strain that exclusively produces this analogue.
CITATION STYLE
Goh, F., Zhang, M. M., Lim, T. R., Low, K. N., Nge, C. E., Heng, E., … Ng, S. B. (2020). Identification and engineering of 32 membered antifungal macrolactone notonesomycins. Microbial Cell Factories, 19(1). https://doi.org/10.1186/s12934-020-01328-x
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