Test of Antibacterial Activity 70% Ethanol Extract of Seeds Black Pepper (Piper Nigrum L) against Bacteria Escherichia Coli Atcc 25922

Abstract

Seeds of black pepper (Piper nigrum L) had antimicrobial compounds was efficacious for treating diarrhea. This purpose of research to determine the activity of the of black pepper seed extract in varying concentrations. Seeds of black pepper extracted by maceration method. Black pepper seeds of 70% ethanol extract made with the series a concentration of 100 mg / ml; 50 mg / ml; 25 mg / ml; 12,5mg / ml; 6.25 mg / ml; 3.125 mg / ml; 1.565 mg / ml; 0.781 mg / ml; 0.3905 mg / ml; negative control, and positive control and cotrimoxazole as controls for comparison. Tests conducted on the bacteria Escherichia coli ATCC 25 922 in dilution. Antibacterial test results showed black pepper seeds extract had an antibacterial activity against Escherichia coli ATCC 25922 to minimum bactericidal concentration (MBC) was 50 mg / ml. Antibacterial activity found on 50 mg/ml of minimum concentration because black pepper seeds extract contained flavonoids, alkaloids, tannins, saponins, essential oils, and piperine. Piperine was a chemical content that highest.