Double-sided micropillar arrays for cell contraction force mapping using optical moirÉ method

Abstract

The mapping of cellular traction forces is fundamental to deeply understanding a wide range of cellular functions and behaviors. The moiré phenomenon has been leveraged as a visualization tool for cellular force mapping, yielding both a magnification effect as well as the capacity for whole-field force measurements. This paper reports the development of a double-sided micropillar array (DMPA) made of PDMS for use in moiré-based cellular force analyses. Herein, the fabricated DMPAs were validated by mapping neonatal rat ventricular myocyte (NRVM) contraction forces. The advantages of the DMPA-based approach yield an improved moiré contrast and enable efficient moiré pattern generation.