Extract Bee Propolis (Trigona sp) for Preventive Increase Protease Activity and Defect of Trachea Histology in Rats (Rattus norvegicus) Exposed to Cigarette Smoke

Abstract

Environmental tobacco smoke (ETS) refers to exposure to tobacco smoke, not from our smoking but from being exposed to someone else's cigarette. Breathing in ETS is known as passive smoking. Cigarette smoke contains dangerous components, include Radical Oxygen Species. Exposure free radical from cigarette result unbalance antioxidant in our body, it can increase protease activity and cell defect. Free radical was inhibited by exogen antioxidants in derivate caffeic acid phenetyl ester (CAPE) propolis Trigona sp. Propolis extract is honey bee product that has many active ingredients with many important roles, as antioxidant, anti-inflammation, DNA protection, and immunomodulator. This research to learn about potential extract propolis Trigona sp for inhibiting increased protease activity and defect of trachea histology in rats exposed to cigarette smoke. It is used five groups, they are negative control, positive control exposed to 2 cigarettes/day, and three preventive groups given by propolis, dose 10, 20 and 30 mg/200gBW/day. We conclude that propolis has the effect to prevent increase protease activity and defect of trachea histology from exposure to cigarette smoke.