Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

T. H. Wu; Y. Chen; S. Y. Park; J. Hong; T. Teslaa; J. F. Zhong; D. Di Carlo; M. A. Teitell; P. Y. Chiou

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Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (2012) 1097-1100

DOI: 10.1109/MEMSYS.2012.6170196

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Abstract

We report a high speed and high purity pulsed laser triggered fluorescence activated cell sorter (PLACS) with a sorting throughput up to 20,000 mammalian cells/s with 37% sorting purity, 90% cell viability in enrichment mode, and >90% purity in high purity mode at 1,500 cells/s or 3,000 beads/s. Fast switching (30 μs) and a small perturbation volume (∼90 pL) is realized by a unique sorting mechanism in which explosive vapor bubbles are generated using focused laser pulses in a single layer microfluidic PDMS channel. © 2012 IEEE.

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Wu, T. H., Chen, Y., Park, S. Y., Hong, J., Teslaa, T., Zhong, J. F., … Chiou, P. Y. (2012). Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 1097–1100). https://doi.org/10.1109/MEMSYS.2012.6170196

Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

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