Isolation and identification of Vibrio parahaemolyticus from seawater and sediment samples in the southern coast of the Caspian Sea

Abstract

The objectives of this study were to investigate the occurrence of Vibrio parahaemolyticus in the seawater and its sediment by molecular techniques and conventional microbiological methods. Of 300 samples analyzed, 20.3 % was recorded positive for V. parahaemolyticus. Of the 62 strains isolated, 26 (8.3 %) were obtained from the seawater samples, and 36 (12 %); from sediments. Only three strains (4.83 %) showed hemolytic activity in Wagatsuma agar. The results of this study demonstrated the presence of V. parahaemolyticus in the southern coast of the Caspian Sea (Northern Iran). Furthermore, the PCR approach proved useful for reliable confirmation of species identification. V. parahaemolyticus is an important human pathogen responsible for food-borne gastroenteritis worldwide. These findings indicated the potential sanitary risk associated with the presence of pathogenic V. parahaemolyticus in the Caspian Sea. © 2012 The Author(s).