Development of microsatellite markers from genomic DNA of Parashorea malaanonan (Dipterocarpaceae) using next-generation sequencing

Abstract

Twenty polymorphic microsatellite markers were developed, using Next Generation Sequencing (Illumina), from genomic DNA of Parashorea malaanonan, a species of the Dipterocarpaceae which is ecologically and economically important in the Philippines. Thirty adult trees from a natural population were used to assess the success of PCR amplification and the degree of polymorphism. The number of alleles per locus varied from three to 13, and observed and expected heterozygosity varied from 0.200 to 0.808 and from 0.301 to 0.890 respectively. Total exclusion probabilities for the first and second parents over the 20 loci were 0.99932499 and 0.99999723 respectively. The high level of polymorphism at these loci makes it possible to obtain precise estimates of genetic parameters and thus the markers will help in studies on population genetics, conservation genetics, and molecular ecology of P. malaanonan.