N-terminal protein modification through a biomimetic transamination reaction

Abstract

(Chemical Equation Presented) One hit wonder: A biomimetic transamination reaction has been developed that employs pyridoxal-5-phosphate to modify the N terminus of proteins and peptides under mild conditions. This technique introduces a uniquely reactive carbonyl group in a single location (see scheme), thus allowing further elaboration through oxime or hydrazone formation. This modification strategy is also compatible with proteins containing a free cysteine residue. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.