Cardioprotective effects of dietary flaxseed post‐ infarction are associated with changes in microrna expression

Abstract

MicroRNAs (miRNAs/miRs) such as miR‐1, miR‐133a, miR‐133b, miR‐135a, and miR‐29b play a key role in many cardiac pathological remodeling processes, including apoptosis, fibrosis, and arrhythmias, after a myocardial infarction (MI). Dietary flaxseed has demonstrated a protective effect against an MI. The present study was carried out to test the hypothesis that dietary flaxseed supplementation before and after an MI regulates the expression of above‐mentioned miRNAs to produce its cardioprotective effect. Animals were randomized after inducing MI by coronary artery ligation into: a) sham MI with normal chow, b) MI with normal chow, and c–e) MI supplemented with either 10% milled flaxseed, or 4.4% flax oil enriched in alpha‐linolenic acid (ALA), or 0.44% flax lignan secoisolariciresinol diglucoside. The feeding protocol consisted of 2 weeks before and 8 weeks after the surgery. Dietary flax oil supplementation selectively upregulated the cardiac expression of miR‐133a, miR‐135a, and miR‐29b. The levels of collagen I expression were reduced in the flax oil group. We conclude that miR‐133a, miR‐135a, and miR‐29b are sensitive to dietary flax oil, likely due to its rich ALA content. The cardioprotective effect of flaxseed in an MI could be due to modulation of these miRNAs.