Review: Factors affecting mass propagation of Vanda orchid in vitro

Abstract

Abstract. Setiaji A, Annisa RRR, Santoso AD, Kinasih A, Riyadi ADR. 2021. Review: Factors affecting mass propagation of Vanda orchid in vitro. Cell Biol Dev 5: 51-62. For the past decade, Vanda has been the primadonna of ornamental orchids in the south and southeast Asia, along with Phalaenopsis and Dendrobium. Along with the increase in demand for Vanda, this genus has faced several threats, from illegal collection to habitat loss. Therefore, mass propagation through in vitro culture is a promising strategy to ensure sustainable business in horticulture and conservation. This review provides an overview and synthesizes various Vanda in vitro culture literature. We showed the researchers' preferences on several aspects for growing Vanda, including species, basal medium, plant growth regulators, explant, and culture conditions. The most commonly used as explants are seeds or protocorms, growing on Murashige & Skoog or Vacin & Went medium. This medium can be added banana homogenate to increase its nutritional value. Vanda seedlings can be incubated at 25 ± 1-3oC, with a lighting intensity of 50 ?mol m-2 s-1 at 12/16 h PP. Choosing a medium that is cheaper but still rich in nutrients and its additives, especially during the subculture phase; selecting explants that are responsive and minimizing the possibility of contamination; as well as seeing the target market in particular, can make Vanda's propagation efforts more effective, efficient, and profitable.