Characterization of Halophilic Isolates Producing Bioactive Metabolites Against Pathogens

Abstract

Halophiles are salt loving microorganisms that are able to survive in high salt conditions. Halophiles are highly diverse group belongs to all three domains of life, Archaea, bacteria and Eucarya. Halophiles adapted themselves through various strategies to maintain their osmotic balance in order to survive in high salt concentrations. Likewise halophiles are also able to produce bioactive molecules such as carotenoids, polyhydroxy-alkanoates, ectoine, bioplastics, and enzymes that have commercial importance. In current study halophiles were isolated at 1M NaCl concentration and characterized, and check its antibacterial activity against pathogenic E.coli, Pseudomonas aeruginosa and Klebsiella pneumonae. Two halophilic strains showed activity against pathogenic E.coli and Klebsiella pneumonae only. Antimicrobial activity was further analyzed through disc diffusion method and by using extracted filtrate. The activity only observed against E.coli after 48 hours old cultures that were served as filtrate extracts and discs against. Furthermore optimization as well as detailed characterization of the secondary metabolites will be needed to find the source of antibacterial metabolites against specific pathogens.