Viro‐immunological characterization of naïve patients with high cerebrospinal fluid (CSF) HIV RNA

Abstract

BACKGROUND: HIV can spread into the central nervous system (CNS) early in the course of infection and this turns into intrathecal inflammation and neuronal damage. We aimed to investigate clinical and immunological parameters associated with elevated CSF VL in HIV-infected ART-naive patients. MATERIALS AND METHODS: HIV+ ART-naive patients underwent a comprehensive battery of neurocognitive (NC) tests and lumbar puncture (LP) for CSF HIV-RNA detection. Plasma HIV-RNA and peripheral T-cell immune-phenotypes (CD38/CD45RA/CD45R0/CD127 on CD4/CD8) were also assessed (flow cytometry). High-CSF HIV RNA was defined as>/=10000cp/mL (H-CSF), while CSF HIV RNA<10000cp/mL characterized low VL patients (L-CSF). Chi-square and Mann-Whitney tests were used. Parameters independently associated with CSF VL were explored by multivariate regression. RESULTS: A total of 131 patients were retrospectively enrolled. Forty-two patients (32%) had CSF VL >10000 cp/mL. CONCLUSIONS: We hereby describe a 32% prevalence of H-CSF in a cohort of HIV+ ART-naive patients. Subjects with high-CSF viral replication are mostly with higher systemic immune activation, in particular the percentage of naive CD8 T-cell is positively associated with CSF VL, irrespective of plasma VL. In HIV+ ART-naive patients, especially if featuring a hyperactivated T-cell immune-phenotype, lumbar puncture should be considered to further guide CNS-targeted cART.