Antibacterial activity of ecofriendly biologically synthesized copper oxide nanoparticles

Abstract

Copper oxide nanoparticles (CuONPs) were prepared using ecofriendly simple method that employs the extract of Syzygium Aromaticum (clove buds) as a reducing and stabilizing agent. The prepared CuONPs were characterized by FT-IR, SEM and EDS techniques to study their morphological and compositional properties. Fourier transform infrared spectroscopy (FTIR) spectrum indicates the presence of various functional groups at different positions. Morphological studies were carried out to study the shape of the synthesized nanoparticles. Energy dispersive X-ray spectroscopy (EDX) shows highly intense peaks of copper (Cu) and oxygen (O), while less intense peaks of carbon (C) and sulfur (S) elements due to the presence of biomolecules of bud extract used in CuONPs formation. The antibacterial activity of the synthesized CuONPs against gram - ve microorganisms viz. E-Coli, Salmonella and Enterobacter bacteria was studied. The produced CuONPs show resistance to the three types of bacteria with greatest resistance to E. coli followed by Salmonella and then Enterobacter.