Lactobacillus alvi sp. nov., isolated from the intestinal tract of chicken

Abstract

Strain R54 T was isolated from the gizzard of hens. The isolate was Gram-positive, facultative anaerobic, gas-forming, catalase-negative, nonmotile, nonspore-forming and short-rod-shaped. The optimal temperature for growth was 40 °C and the DNA G+C content was 42.7 mol%. The 16S rRNA gene sequences similarity showed that strain R54 T was most closely related to Lactobacillus ingluviei LMG 20380 T (97.5%), followed by Lactobacillus coleohominis CIP 106820 T (96.1%), Lactobacillus secaliphilus DSM 17896 T (95.6%) and Lactobacillus gastricus LMG 22113 T (95.4%). The DNA-DNA relatedness between strain R54 T and L. ingluviei LMG 20380 T, was 43.3%. The predominant cellular fatty acids of strain R54 T were C 18:1 ω9c (64.9%) and C 16:0 (20.0%), and the major polar lipid group was phospholipids. On the basis of polyphasic taxonomy approach, strain R54 T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus alvi sp. nov. is proposed (type strain R54 T = KCCM 90099 T = JCM 17644 T). © 2011 Federation of European Microbiological Societies.