Identifikasi Genetik Udang Mantis Dengan Pendekatan DNA Barcoding Gen Sitokrom Oksidase 1 (CO1)

Abstract

Mantis shrimp is one of the marine organisms that has a very abundant diversity of species. The similarity of morphology of this group make it difficult to identify morphologically. Therefore, identification was carried out using a DNA barcoding approach. The purpose of this study was to identify the mantis shrimp species from Manokwari waters using molecular techniques. The CO1 gene fragments were amplified by using LCO and HCO primers. The DNA sequences obtained were analyzed for homology, phylogeny, and genetic distance using MEGA X software. A total of 537 base pairs of CO1 gene fragments of mantis shrimp from Manokwari were obtained. The results of the homology analysis on GenBank, show the highest similarity of 91.18% with sequences from the genus Gonodactylus. Analysis of phylogenetic tree and genetic distance showed the same results and supported each other with homology analysis on GenBank data.