Effect of Chattonella Exposure on Acid-Base Status of the Yellowtail

Abstract

The effect of Chattonella exposure on the acid-base status of the yellowtail Seriola quinqueradiata was analyzed to assess the validity of the hypothesized mechanism of fish kill by this plankton species. Based on the finding that gill carbonic anhydrase was inactivated in fish exposed to Chattonella, it was hypothesized that this inactivation resulted in certain physiological disorders such as decreases in blood pH and Po2. None of the acid-base variables measured in this study (arterial blood pH (pHe), red blood cell intracellular pH (pHi), Pco2 (Paco2), and total C02 of plasma (Taco2p)) varied substantially from their control values after the onset of exposure except for some increases in pHi. It was only the terminal blood samples that demonstrated sharp decreases in pHe and pHi and elevation of Paco2. In contrast, arterial Po2 decreased rapidly after the onset of plankton exposure. Overall these results do not support the above hypothesis. © 1991, The Japanese Society of Fisheries Science. All rights reserved.