Synthetic community with six Pseudomonas strains screened from garlic rhizosphere microbiome promotes plant growth

Abstract

The rhizosphere microbiome plays an important role in the growth and health of many plants, particularly for plant growth-promoting rhizobacteria (PGPR). Although the use of PGPR could improve plant production, real-world applications are still held back by low-efficiency methods of finding and using PGPR. In this study, the structure of bacterial and fungal rhizosphere communities of Jinxiang garlic under different growth periods (resume growth, bolting and maturation), soil types (loam, sandy loam and sandy soil) and agricultural practices (with and without microbial products) were explored by using amplicon sequencing. High-efficiency top-down approaches based on high-throughput technology and synthetic community (SynCom) approaches were used to find PGPR in garlic rhizosphere and improve plant production. Our findings indicated that Pseudomonas was a key PGPR in the rhizosphere of garlic. Furthermore, SynCom with six Pseudomonas strains isolated from the garlic rhizosphere were constructed, which showed that they have the ability to promote plant growth.