Wound healing activities of Moringa oleifera leaves extract cultivated in Kurdistan region-Iraq

Abstract

Medicinal plants have been used for many years as an ancient curative method for treating and healing wounds in different cultures. Therefore, and accordingly this work has been conducted to study wound healing activity of Moringa oleifera leaves extract cultivated in the Kurdistan region in the northern Iraq (KRG). In the current investigation, experimentally-induced wounds in rats have been infected with strains of Staphylococcus aureus and Pseudomonas aeruginosa clinically isolated from the wound site in hospitalized patients. High Performance Liquid Chromatography (HPLC) is used to determine some bioactive compounds within the extract. Plant leaf was extracted by using the maceration method and 70% ethanol as solvent. The HPLC results were dependent on comparison between the extract with standards. Two lacerated wounds were made on each animal at either side of the thoracolumbar spine and inoculated by a 0.4 ml bacterial suspension. The treatment regimen was for 14 days with different formulation of ethanolic leaves extract, and gentamicin ointment as a control positive. At the end point of the experimental trial, animals were euthanized humanly at day 15. Samples from healed-wounded skin was prepared for histological evaluation. Generally speaking, our findings indicated that alcoholic leaf extract showed potential wound healing property in different concentration as a dose-dependent manner of the extracted ointment 3.5%, 5%,10% particularly 10% of the extract formulation which showed better results in comparison to gentamicin ointment. The presented essential constituents for Moringa leaf derivatives were gallic acid (3461 ppm), catechin (1201 ppm), rutin (286 ppm) and quercetin (88 ppm). Last but not least, the extract was able to provide promising evidence to possess a drug formulation material.