Determination of Perfluorochemicals in Organs and Body Fluids by Gas Chromatography

Abstract

A gas chromatographic method for the quantitative determination of perfluoro-chemicals (FCs) contained in organs and body fluids was presented. Following destruction of FC emulsion by addition of ethanol, FCs were extracted with 1,1,2-trichlorotrifluoro-ethane and applied to a gas chromatograph equipped with a hydrogen flame ionization detector. A column (2 m × 4 mm i.d.) of 20% Silicone OV-17 on Chromosorb W AW (60–80 mesh) was used at 60°. The retention time of each FC peak was found between 0.5 and 1.0 min. Complete separation of FC from the solvent was achieved and any undesirable, interfering peaks were not detected on the gas chromatogram. The determination of their contents was carried out by calculation of peak height ratio of them to benzotrifluoride added as the internal standard. Recovery of four kinds of FCs by this method ranged from 94.7 to 102.6% when known amount of respective FCs in emulsified form was added to the organs or blood with the standard deviation of ±3.3%. © 1975, The Pharmaceutical Society of Japan. All rights reserved.