In vitro platelet adhesion of PNaAMPS/PAAm and PNaAMPS/PDMAAm double-network hydrogels

Abstract

Nowadays, tough double-network (DN) hydrogels have attracted great attention owing to their excellent mechanical properties and good biocompatibility, which give them the potential to be used as blood-contacting soft tissue prostheses and medical devices. However, the study of platelet adhesion behavior on the surface of DN hydrogels has not been reported yet. In this work, the human platelet adhesion on the surface of poly (sodium 2-acrylamido-2-methyl-propanesulfonate) PNaAMPS/poly acrylamide (PAAm) and PNaAMPS/poly (N,N′-dimethylacrylamide) (PDMAAm) DN hydrogels is investigated under static conditions in vitro. The numbers of adherent platelets on PNaAMPS/PAAm and PNaAMPS/PDMAAm hydrogels are 16 ± 7 and 9 ± 8 cells per 104 μm2, respectively, which are far less than 297 ± 41 cells per 104 μm2 on polyethylene terephthalate (PET) and 187 ± 26 cells per 104 μm2 on negatively charged PNaAMPS (4 mol%) hydrogel. The results indicate the excellent antiplatelet performance of DN hydrogels. Moreover, the platelet adhesion mechanism is also discussed. The platelet adhesion is affected by the chemical component, zeta potential, and serum proteins adsorption of the hydrogel. Human platelet adhesion on the surface of double-network (DN) hydrogels is investigated under static conditions in vitro. The numbers of adherent platelet on poly (sodium 2-acrylamido-2-methyl-propanesulfonate) (PNaAMPS)/poly acrylamide (PAAm) and PNaAMPS/poly (N,N'-dimethylacrylamide) (PDMAAm) hydrogels are far less than that on the polyethylene terephthalate (PET) and the negatively charged PNaAMPS hydrogels. The platelet adhesion is affected by the chemical component, zeta potential, and serum proteins adsorption of the hydrogel.