Abstract
A protocol is described to purify small (s)RNA molecules from tissues of grapevine and other woody plants. The protocol has been specifically developed to analyze sRNA populations by high-throughput sequencing. It has been widely used on species of the genera Prunus and Vitis particularly rich in polyphenols and other enzyme-inhibiting compounds. The high quality of the sRNAs extracted from leaf or phloem tissues makes them suitable for all molecular biology reactions, in particular for next-generation sequencing library preparation.
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Giampetruzzi, A., Chiumenti, M., Minafra, A., & Saldarelli, P. (2018). Small RNA isolation from tissues of grapevine and woody plants. In Methods in Molecular Biology (Vol. 1746, pp. 27–36). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7683-6_2
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