RETRACTED: Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart

Abstract

D‐galactose (D‐gal) administration causes oxidative disorder and is widely utilized in aging animal models. Therefore, we subcutaneously injected D‐gal at 200 mg/kg BW dose to assess the potential preventive effect of thymoquinone (TQ) and curcumin (Cur) against the oxidative alterations induced by D‐gal. Other than the control, vehicle, and D‐gal groups, the TQ and Cur treated groups were orally supplemented at 20 mg/kg BW of each alone or combined. TQ and Cur effectively suppressed the oxidative alterations induced by D‐gal in brain and heart tissues. The TQ and Cur combination significantly decreased the elevated necrosis in the brain and heart by D‐gal. It significantly reduced brain caspase 3, calbindin, and calcium‐binding adapter molecule 1 (IBA1), heart caspase 3, and BCL2. Expression of mRNA of the brain and heart TP53, p21, Bax, and CASP‐3 were significantly downregulated in the TQ and Cur combination group along with upregulation of BCL2 in comparison with the D‐gal group. Data suggested that the TQ and Cur combination is a promising approach in aging prevention.