Epoxy functionalized iron oxide magnetic nanoparticles for catalase enzyme covalent immobilization

Abstract

An aqueous solution of magnetite (Fe3O4) nanoparticles was synthesized using the method of co-precipitation. The nanoparticles were activated with epichlorohydrin for covalently immobilizing the catalase enzyme. The immobilization conditions were optimized as 0.07 mg/ml catalase for 1 h contact time. The properties of free and immobilized catalase were also investigated. The immobilized enzyme showed enhanced activity at alkaline pH and retained about 90% of its relative activity between pH (6–8) and resisted the high temperature and retained 90% of its relative activity at 50 °C. Kinetic parameters of free and immobilized catalase were investigated. While the Vmax value of the immobilized enzyme was reduced 2.4 fold compared to the free enzyme, the KM value of the immobilized catalase was higher by 2.2 fold than the free enzyme. The formulated matrix enhanced the velocity of the immobilized catalase more than the free one and was able to be used for about 18 cycles with retention of 80% of its activity. The immobilized catalase on epoxy functionalized iron oxide nanoparticles is promising as a nano-bio-catalyst carrying out in many industries and different fields.