Cytotoxicity assessment of metal and 3d printed resin orthodontic attachments an in vitro cell culture behavior study

Abstract

Materials used in orthodontics are constantly changing and improving, but biocompatibility remains one of their most important characteristics.The purpose of this study is to evaluate the in vitro cellular behavior of dental MSC cells when put in contact with metal and 3D printed resin orthodontic attachments.Cell monolayers were seeded in wells and left to adhere. Polymer and metal orthodontic attachments were placed into the wells. The cells were analyzed on an inverted light microscope. The cellular viability was determined using Alamar Blue assay. Results showed that the morphology and the density of cells are not affected by the presence of either orthodontic material compared to the controls.The metabolic activity of the cells was not altered. Similar cellular reactions,were observed in contact with both materials used in our short term in vitro study.