β-aminobutyric acid does not induce defenses or increase Norway spruce resistance to the bluestain fungus Grosmannia penicillata

Abstract

Priming of Norway spruce (Picea abies) inducible defenses is a promising way to protect young trees from herbivores and pathogens. Methyl jasmonate (MeJA) application is known to induce and potentially prime Norway spruce defenses but may also reduce plant growth. Therefore, we tested β-aminobutyric acid (BABA) as an alternative priming chemical to enhance spruce resistance, using 2-year-old Norway spruce plants. We compared inducible defense responses, i.e. traumatic resin duct formation and accumulation of defensive metabolites, in bark and xylem tissues of BABA- or MeJA-treated plants before and after wounding. We also evaluated the effect of these chemical treatments on Norway spruce resistance to the pathogenic bluestain fungus Grosmania penicilliata. BABA did not induce defense responses or pathogen resistance, it even reduced concentrations of total terpenes in the treated plants. In contrast, MeJA induced traumatic resin duct formation, accumulation of flavonoids, pathogen resistance, and did not affect plant growth. For the first time, flavan-3-ols (catechins) were shown to have a primed response to MeJA treatment in Norway spruce. Our results indicated that BABA is not a suitable alternative priming chemical to MeJA in Norway spruce.