The potential of VipAlbumin® to chronic inflammation in type 2 diabetes mellitus Balb/C mice model

Abstract

Diabetes Mellitus (DM) is one of diseases which have increasing number of sufferers every year. Almost all DM patients are type 2 DM. One of the causes of type 2 DM is a chronic inflammation due to an increase of circulating proinflammatory cytokines such as TNF-a, IFN-gand IL-6. VipAlbumin® from snakehead fish extract is expected to be useful as an alternative treatment of type 2 DM because of its proinflammatory activity. The aim of this study was to determine the effect of VipAlbuminR on regulatory T cell activation, macrophage cells, proinflammatory cytokines and NF-κB. The experiments were done by inducing mice model of type 2 DM with STZ 100 mg/kg BW and then gave them oral administration of VipAlbumin®0 mg/g BW, 0.01664 mg/g BW, 0.0416 mg/g BW and 10.4 mg/g BW for 14 days. The data were statistically analyzed with one way ANOVA with significance value 0.05% and Tukey test using SPSS version 16 for Windows. The results showed the decreasing number of regulatory T cells in type 2 DM mice, increasing number of macrophage cells and proinflammatory cytokines TNF-a, IFN-gand IL-6 as well as the increasing number of NF-κB as a transcription factor of inflammatory mediators in CD4+ T cells, CD8+ T cells and macrophages (CD68 +) compared to healthy mice (p<0.05). Oral administration of VipAlbuminR for 14 days was proven to cure type 2 DM mice (K +) by increasing the number of regulatory T cells, decreasing the number of macrophage cells and proinflammatory cytokines TNF-a, IFN-gand IL-6 and inhibiting NF-κB in T lymphocytes CD4 +, CD8+ and macrophages at the level equivalent to healthy mice and significantly different (p<0.05) compared with K+ group.