Study on GC-MS fingerprint analysis in rhizome of volatile oil of Acorus tatarinowii

Abstract

Objective: To establish the method of fingerprint analysis on volatile oil in rhizome of Acorus tatarinowii by GC-MS, and to study the main characteristic components. Method: The main components of 10 samples were determined by GC-MS. Result: The injector temperature was 250°C. The interface temperature was 230°C. The column flow was 1.3 mL·min-1. The column pressure was 80 kPa. The detector volt was 1.4 kV. The temperature rate was 3°C·min-1. And the main characteristic components were composed of the methyleugenol (2.13%), cis-methylisoeugenol (4.48%), trans-methylisoeugenol (0.82%), γ-asarone(4.51%), β-asarone(66.15%), α-asarone (6.35%). And the RSD of precision and reproducibility and stability was almost in the range of 5%. Conclusion: The method is reliable, accurate and can be used for fingerprint analysis of volatile oil of Acorus tatarinowii.