Rapid and specific identification of Yersinia pestis by using a nested polymerase chain reaction procedure

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Abstract

We developed a 4-h nested polymerase chain reaction assay that detected a region of the plasminogen activator gene of Yersinia pestis in 100% of 43 Y. pestis strains isolated from humans, rats, and fleas yet was unreactive with the closely related species Yersinia enterocolitica and Yersinia pseudotuberculosis.

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Campbell, J., Lowe, J., Walz, S., & Ezzell, J. (1993). Rapid and specific identification of Yersinia pestis by using a nested polymerase chain reaction procedure. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.31.3.758-759.1993

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